The evaluation of the site as a whole should focus more on its behavior as a unit than on the details of page layout, which you have already thoroughly considered when designing the template page.
Ensure that your site works as intended:
Test every link.
Check that each page's position in the site structure is obvious to the user.
Make sure all images display.
Make sure all special characters display properly.
Make sure alt values for all images are descriptive and sensible - view your site without images to test this performance.
Test the site in different browsers.
Read your entire site to ensure you haven't introduced any mistakes.
Test all user input aspects of your site.
Remember: you created your site so you understand it, but you aren't the intended audience of your site. You must consider how a first time user will cope with the experience you are providing in order for your site to be successful.